Nicotinamide Riboside Prevents and Enhances Therapy in Pancreatic Ductal Adenocarcinoma: Preclinical Findings

Synopsis Pancreatic ductal adenocarcinoma (PDAC) shows progressive loss of SIRT3, a mitochondrial deacetylase whose downregulation correlates with poor patient survival. Using spontaneous and expe...

Mechanistic Insights into NAD+ Driven Activation of Phosphite Dehydrogenase: Preclinical Findings

Synopsis Research shows that the energy from the ADP part of the NAD+ molecule helps drive a shape change in phosphite dehydrogenase (PTDH), which activates the enzyme to transfer a hydride from p...

Impact of Energy Production Proteins and Molecules in Triple-Negative Breast Cancer: Preclinical Findings

Synopsis Triple-negative breast cancer (TNBC) is a very aggressive cancer with few treatment options and a high ability to spread. This study found that TNBC cells use a process in their mitochond...

Mixed-Mode LC-MS Method Quantifies NAD+ and Its Metabolites: Preclinical Findings

Synopsis Measuring NAD+ and its metabolites is technically difficult because these molecules are highly polar and poorly retained by standard chromatography. This study developed a robust mixed-mo...

Nicotinamide Riboside and NMN Boost NAD+ Through Microbiota Conversion: Preclinical Findings

Synopsis Using NAD+ metabolomics in mice, this study found that nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) are not directly absorbed intact in large amounts. Instead, most or...

CD38 Converts NMN to NaMN Through Base Exchange: Preclinical Findings

Synopsis Nicotinamide mononucleotide (NMN) is generally assumed to boost NAD+ by feeding directly into the canonical salvage pathway. However, this study shows that NMN treatment unexpectedly incr...

NR and Inosine Restore Metabolic Activity in NAXD-Deficient Cells: Preclinical Findings

Synopsis Mutations in the metabolite-repair enzyme NAXD, which normally converts damaged NADHX/NADPHX back into functional cofactors, cause the severe neurometabolic disorder PEBEL2. In human NAXD...

Standardized Assay Quantifies NAD+ and NADH in Worms, Mice, and Human Blood: Preclinical Findings

SynopsisA standardized assay was developed to measure NAD+, NADH, and the NAD+/NADH ratio across multiple biological samples, including C. elegans, mouse muscle, mouse blood, and human blood. The p...

Nicotinamide Riboside Inhibits CD38 in Living Cells: Preclinical Findings

Synopsis Nicotinamide adenine dinucleotide (NAD+) is essential for energy production, metabolism, and cell signaling, but its levels naturally decline with age and disease. One key reason for this...

Altering the NAD+/NADH Ratio in Reprograms the Metabolome in Glioblastoma: Preclinical Findings

Synopsis Glioblastoma multiforme (GBM) is the most aggressive and deadly form of brain cancer, known for its rapid growth and invasion into surrounding tissue. Because GBM cells rewire their metab...

Fluorinated NR Enhances NAD+ Depletion in Glioblastoma Therapy: Preclinical Findings

Synopsis Glioblastoma is one of the most aggressive and treatment-resistant brain cancers, partly because its cells rely heavily on NAD+, a molecule vital for energy production and survival. The d...

NAD+ Precursor Supplementation Enhances Metabolic Efficiency and Antibody Production: Preclinical Findings

Synopsis This study explored how NAD+ precursors can enhance productivity in Chinese hamster ovary (CHO) cells, which are widely used to produce therapeutic antibodies. Previous research showed th...

Effects of Nicotinamide Riboside on Lung Cells: Preclinical Findings

Synopsis This study examined how different concentrations of nicotinamide riboside (NR)—a vitamin B3–derived NAD+ precursor—affect human lung cells grown in both 2D and 3D cultures. Researchers fo...

Targeting Autophagy-NAD Pathway Protects Cells in Niemann-Pick Type C1 Disease: Preclinical Findings

SynopsisImpaired autophagy, which leads to the buildup of misfolded proteins and damaged organelles, has been linked to many human diseases. Loss of autophagy in metabolically active cells can caus...

Comparative Analysis of Nicotinamide Mononucleotide and Nicotinamide Riboside in Protecting HeLa Cells From Cisplatin-Induced DNA Damage: Preclinical Findings

Synopsis Cisplatin, a common chemotherapy drug, works by damaging DNA in cancer cells—but it can also harm healthy cells, leading to side effects. This study compared two vitamin B3–derived NAD+ p...

NAMPT Inhibition in Neuroendocrine Cancers and the Role of NAD+ Precursors: Preclinical Findings

Synopsis Nicotinamide phosphoribosyltransferase (NAMPT) is a key enzyme that helps cancer cells make NAD+, a molecule essential for energy production and survival. Drugs that block NAMPT (NAMPT in...

Nicotinamide Riboside and Phycocyanin Oligopeptides Reduce Stress-Induced Cellular Aging: Preclinical Findings

Synopsis Chronic stress accelerates cellular aging and increases inflammation and oxidative damage, but certain nutrients may help protect against these effects. In this study, researchers tested ...

Nicotinamide Riboside Defends Against Sulfur Mustard-Related Toxicity: Preclinical Findings

Synopsis Sulfur mustard (SM) and related chemicals are toxic compounds that damage DNA by depleting NAD+, a molecule essential for DNA repair and energy balance. This study examined whether restor...

Effects of Increasing NAD+ on the Pluripotency of Stem Cells: Preclinical Findings

Synopsis Nicotinamide adenine dinucleotide (NAD+) is vital for cellular energy and signaling, and recent studies suggest it may influence how embryonic stem cells maintain their ability to become ...

Nicotinamide Riboside Protects Against Ionizing Radiation-Induced Aging: Preclinical Findings

Synopsis Radiation therapy effectively kills cancer cells but can also damage healthy tissues, leading to premature aging and long-term side effects in survivors. In this study, researchers used a...