Article: Effects of Increasing NAD+ on the Pluripotency of Stem Cells: Preclinical Findings

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Cellular Health Preclinical

Effects of Increasing NAD+ on the Pluripotency of Stem Cells: Preclinical Findings

Synopsis

Nicotinamide adenine dinucleotide (NAD+) is vital for cellular energy and signaling, and recent studies suggest it may influence how embryonic stem cells maintain their ability to become any cell type (pluripotency). In this study, researchers examined how changes in NAD+ levels affected mouse embryonic stem cells (E14 cells). Increasing NAD+ with nicotinamide riboside (NR)—a vitamin B3 derivative—raised intracellular NAD+ by about 20%, but did not alter pluripotency markers like Oct4 or stem cell characteristics. Even when NAD+ production was severely reduced using an inhibitor (FK866), the cells maintained pluripotency, though Oct4 levels slightly decreased. The findings suggest that embryonic stem cells can preserve their pluripotent state even when NAD+ metabolism fluctuates, highlighting their resilience and metabolic flexibility.

Journal

Cell and Tissue Biology

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